RT-qPCR Probes Master Multiplex com UDG | Detecção por Probes tipo TaqMan® - Cellco
Kit de transcrição reversa em tempo real otimizado para detecção de RNA viroses

For in vitro use only!

Envio: 
Shipped on blue ice

Condições de armazenamento: 
Store at -20 °C in dark
Avoid freeze/thaw cycles

Validade: 
12 months

Forma: 
Liquid

Concentração:
Master Mix 2x concentrated

Descrição:
RT-qPCR Probes Master Multiplex is designed for quantitative reverse transcription real-time analyses of up to 5 RNA targets simultaneously using Dual Labeled Fluorescent Probes (suitable for TaqMan system and other probes). It is a fast, accurate and optimized mix for several approaches.

The kit is based on a genetically engineered reverse transcriptase with enhanced thermal stability providing increased specificity, high cDNA yield and improved efficiency for highly structured and long cDNA fragments and contains all reagents required for RT-qPCR (EXCEPT TEMPLATE, PRIMERS AND FLUORESCENT PROBE) to ensure fast and easy preparation. The premium quality SCRIPT Reverse Transcriptase and Taq DNA Polymersae Hot Start antibody-coupled in a optimized reaction buffer containing ultrapure dNTPs, ensure superior real time PCR results for multiplex reaction.

RT-qPCR Probes Master Multiplex is used to amplify doublestranded DNA from singlestranded RNA templates to allow a rapid real-time quantification of RNA targets. In the reverse transcription step the reverse transcriptase synthesizes singlestranded DNA molecules (cDNA) complementary to the RNA template.

In the first cycle of the PCR step hot-start DNA polymerase synthesizes DNA molecules complementary to the cDNA, thus generating a double-stranded DNA template. The hot-start polymerase activity is blocked at ambient temperature by antibody and switched on automatically at the onset of the initial denaturation.

The thermal activation prevents the extension of non-specifically annealed primers and primer-dimer formations at low temperatures during PCR setup. RT-qPCR Probes Master Multiplex offers tremendous convenience when applied to analysis of targets from multiple samples of RNA and minimizes the risk of contaminations.

Kit Content:
RT-qPCR Probes Master Multiplex 2x (red cap)
Script Reverse Transcriptase, Taq DNA Polymerase Hot Start (antibody-coupled), RNAse Inhibitor, UDG, dNTP mix with dUTP and stabilizers.

RNase-free Water (white cap)

Sensitivity:
Targets can be detected from 1 copy of the genes. The UDG system prevent from cross-contamination which is indispensable for diagnostic detection.