SYBR® Green Fluorescent DNA Stain | Suplementos qPCR - Cellco
Corante intercalante de DNA para detecção de fluorescência em tempo real qPCR

For in vitro use only!

Shipping:
Shipped on blue ice Storage

Conditions:
Store at -20 °C (in the dark)

Shelf Life:
12 months

Form:
Orange liquid, supplied in 20 mM Tris-HCl pH 8.5, 0.1 mM EDTA and 0.01 % Tween-20

Concentration:
100 µM

Spectroscopic Properties:
λ_exc 495 nm (bound to DNA); λ_em 520 nm (bound to DNA)

Description:
SYBR® Green Fluorescent DNA Stain is a superior DNA intercalator dye specially developed for DNA analysis applications including real-time PCR (qPCR). Upon binding to DNA, the non-fluorescent dye becomes highly fluorescent while showing no detectable inhibition to the PCR process. The dye is extremely stable both thermally and hydrolytically, providing convenience during routine handling. SYBR® Green Fluorescent DNA Stain is supplied as 100 µM concentration. Vortex SYBR® Green Fluorescent DNA Stain thoroughly prior to its use. An SYBR® Green concentration of 0.5-1.0 µM in the final assay is recommended. Add SYBR® Green Fluorescent DNA Stain as indicated in the table below per assay. Please note that the preparation of a master mix may be crucial in quantitative PCR reactions to reduce pipetting errors. Select the optical setting for SYBR® Green or FAM on the detection instrument.

Applications:
SYBR® Green Fluorescent DNA Stain is supplied as 100 µM concentration. Vortex SYBR® Green Fluorescent DNA Stain thoroughly prior to its use. An SYBR® Green concentration of 0.5-1.0 µM in the final assay is recommended. Add SYBR® Green Fluorescent DNA Stain as indicated in the table below per assay. Please note that the preparation of a master mix may be crucial in quantitative PCR reactions to reduce pipetting errors. Select the optical setting for SYBR® Green or FAM on the detection instrument.