ROX Reference Dye | Suplementos qPCR - Cellco
Corante de referência para normalização de sinal de fluorescência

For in vitro use only!

Shipping:
Shipped on blue ice Storage

Conditions:
Store at -20 °C (in the dark)

Shelf Life:
12 months

Form:
Liquid, pink,  supplied in 20 mM Tris-HCl pH 8.4, 0.1 mM EDTA and 0.01 % Tween-20

Concentration:
50x conc. (25 µM)

Description:
ROX reference dye is optimized for block-based real-time PCR instruments that are compatible with the evaluation of the ROX reference signal. The dye does not take part in the PCR reaction but allows to normalize for non-PCR related signal variation and provides a baseline in multiplex reactions. The dye is supplied as 50x concentration.

Spectroscopic Properties:
Excitation maximum: λ_exc 576 nm
Emission maximum: λ_em 601 nm

Preparation of the qPCR master mix:
The preparation of a master mix is crucial in quantitative PCR reactions to reduce pipetting errors. Vortex the ROX reference dye thoroughly prior to addition to the master mix. Add 1 µL ROX reference dye for each 50 µL of master mix.