qPCR SybrMaster + ROX | Detecção por SYBR®Green - Cellco
Master mix para PCR em tempo real com corante de DNA SybrGreen® e ROX

For in vitro use only!

Envio:
Shipped on blue ice

Condições de armazenamento:
Store at -20 °C
(Avoid freeze/thaw cycles, store in dark). 

Concentração:
2 x conc.

Validade:
12 months

Forma:
Liquid

Spectroscopic Properties:
λ_exc 494 nm (bound to DNA), λ_em 521 nm (bound to DNA)

Kit contents:
qPCR SybrMaster (red cap)
Antibody-blocked hot start polymerase, dATP, dCTP, dGTP, dTTP, KCl, (NH₄)₂SO₄, MgCl₂, SYBR® Green DNA intercalator dye, additives and stabilizers

ROX reference dye (#PCK-121)
25 µM ROX Reference Dye.

SYBR® Green Fluorescent DNA Stain:
SYBR® Green Fluorescent DNA Stain is a superior DNA intercalator dye specially developed for DNA analysis applications including real-time PCR (qPCR). Upon binding to DNA, the non-fluorescent dye becomes highly fluorescent while showing no detectable inhibition to the PCR process. The dye is extremely stable, providing convenience during routine handling. SYBR® Green is in contrast to EvaGreen® not recommended for high-resolution melting curve analysis (HRM). To perform the SYBR® Green-based assay simply select the optical setting for SYBR® Green on the detection instrument.

Description:
qPCR SybrMaster + ROX is designed for quantitative real-time analysis of DNA samples. The mix contains all reagents required for qPCR (except template and primers) in a premixed 2x concentrated ready-to-use solution. It is recommended for routine PCR applications, high throughput PCR or genotyping and provides an improved specificity and sensitivity when amplifying low-copy-number targets or working with complex backgrounds. The mix is based on an optimized hot-start polymerase. Its activity is blocked by antibody at ambient temperature and switched on automatically at the onset of the initial denaturation. The thermal activation prevents the extension of nonspecifically annealed primers and primer-dimer formation at low temperatures during PCR setup. The fluorescent DNA stain SYBR® Green intercalates into the amplification product during the PCR process and allows the direct quantification of target DNA without the need to synthesize sequence-specific labeled probes (i.g. TaqMan® Probes).