qPCR GoldMaster lowROX | Detecção por EvaGreen para Genotipagem / High Resolution Melt (HRM) - Cellco
Master mix para real-time PCR com corante de DNA EvaGreen, Referencia passiva ROX e GoldTaq hot start polimerase quimicamente modificada

For in vitro use only!

Envio:
Shipped on blue ice

Condições de armazenamento:
Store at -20 °C
(Avoid freeze/thaw cycles, store in dark). 

Concentração:
2 x conc.

Validade:
12 months

Forma:
Liquid

Spectroscopic Properties:
λ_exc 500 nm (bound to DNA); λ_em 530 nm (bound to DNA)

Kit contents:
qPCR GoldMaster low ROX (red cap)
GoldTaq DNA Polymerase, dATP, dCTP, dGTP, dUTP, EvaGreen®, 100 nm ROX, reaction buffer with KCl, (NH₄)₂SO₄, MgCl₂ and stabilizers.

ROX reference dye:
The qPCR GreenMaster low ROX contains 50 nM ROX passive reference dye in the final assay. The dye does not take part in the PCR reaction but allows to normalize for non-PCR related signal variation and provides a baseline in multiplex reactions.

EvaGreen® Fluorescent DNA Stain:
EvaGreen® Fluorescent DNA Stain is a superior DNA intercalator dye specially developed for DNA analysis applications including real-time PCR (qPCR) and high-resolution DNA melting curve analysis (HRM). Upon binding to DNA, the non-fluorescent dye becomes highly fluorescent while showing no detectable inhibition to the PCR process. The dye is extremely stable both thermally and hydrolytically, providing convenience during routine handling. The high quantum yield, excellent stability and lowest inhibition toward PCR makes it the ideal fluorophore in real-time PCR applications and a superior replacement for the ®widely used SYBR Green I dye. ®To perform the EvaGreen-based assay simply select the optical ®setting for SYBR Green or FAM on the detection instrument.

Description:
qPCR GoldMaster low ROX is designed to provide superior specificity and sensitivity for the quantitative real-time analysis ®of DNA samples using the fluorescent DNA stain EvaGreen. The fluorescent dye in the master mix intercalates into the amplification product  and enables the rapid analysis of target DNA without sequence-specific labeled probes. It provides an easy-to-handle and powerful tool for quantification of sample DNA in a broad dynamic range of up to 6 orders of magnitude with exceptional  precision. The qPCR GoldMaster contains all reagents required for qPCR (except template and primer) in a premixed 2x concentrated ready-to-use solution. The high specificity and sensitivity is achieved by an chemically modified hot-start polymerase. Its activity is blocked at ambient temperature and switched on automatically in a hot start initial cycle allowing flexibility in the reaction set up. The thermal activation prevents the extension of nonspecifically annealed primers and primer-dimer formations. The mix contains dUTP instead of dTTP and allows an UNG (UracilN-Glycosylase) treatment to prevent carry-over contaminations of DNA from previous PCR reactions. The mix also contains ROX reference dye.