Assine a Newsletter e receba nossas promoções
Fique por dentro de todos os nossos super descontos e novidades!
Master mix Taq Pol (2X) | Master Mixes - Cellco
Master mix Taq Pol (2X)
Ficha técnica
| Código | Master mix Taq Pol (2X) |
| Categoria | PCR Convencional |
| Marca | Cellco |
Descrição Geral
Master mix Taq Pol (2X) | Master Mixes - Cellco
Master mix de DNA polimerase termoestável
Pronto para uso
For in vitro use only!
Envio: Shipped on blue ice
Condições de armazenamento: Store at -20 °C
Avoid freeze/thaw cycles. Taq Pol – Master mix (2X) is also stable for three months at 4°C, so for frequent use, an aliquot may be kept at 4°C.
Validade: 12 months
Kit contents:
2x Taq Pol Master Mix (violet cap)
Composition: 0.05 U/ µL Taq DNA polymerase, reaction buffer, 0.3 mM MgCl2, 0.4 mM of each dNTP (dATP, dCTP, dGTP, dTTP), and stabilizers.
Composition: 0.05 U/ µL Taq DNA polymerase, reaction buffer, 0.3 mM MgCl2, 0.4 mM of each dNTP (dATP, dCTP, dGTP, dTTP), and stabilizers.
Descrição:
Taq Pol Master Mix contains Taq Polymerase in an optimized PCR buffer with Mg2+ and dNTPs. It contains all reagents required for PCR (except template and primer) in a premixed 2x concentrated ready-to-use solution and should be used at a 1X concentration with DNA template and primers in a total reaction volume of 25 or 50 µL. The Master Mix is recommended for use in routine PCR reactions from templates including pure DNA solutions, bacterial colonies, and cDNA. It is optimized for high specificity and guarantees minimal by-product formation.
PCR Reaction Setup:
The PCR procedure below shows appropriate volumes for a single 50-μL reaction. For multiple reactions, prepare a master mix of components common to all and then dispense appropriate volumes into each PCR reaction tube prior to adding template DNA and primers. Thaw, mix, and briefly centrifuge each component before use.
Add the following components to a microcentrifuge tube:
1. Prepare PCR master mix
Note: Consider the volumes for all components listed next steps to determine the correct amount of water required to reach your final reaction volume.
| Component | 50 μL rxn | Final conc. |
| Water, grade PCR | to 50 μl | - |
| 2 X Taq Pol Master Mix | 25 μl | 1x |
Mix and briefly centrifuge the components.
2. Add template DNA and primers
| Component | 50 μl rxn | final conc. |
| Forward primer (10 μM) | 0,5 – 2,5 μl | 0,1 – 0,5 μM |
| Reverse primer (10 μM) | 0,5 – 2,5 μl | 0,1 – 0,5 μM |
| Template DNA | x μl | 10 pg – 1 μg* |
Cap each tube, mix, and briefly centrifuge the content.
3. Incubate reactions in a thermal cycler
Recommended cycling conditions:
| Initial denaturation | 95 °C | 1 min | 1x |
| Denaturation | 95 °C | 15 - 30 sec | 30 cycles |
| Annealing** | 45 - 68 °C | 15 - 30 sec | 30 cycles |
| Elongation*** | 72 °C | 30 sec - 4 min | 30 cycles |
| Final extension (optional) | 72 °C | 2 min | 1x |
| Hold | 4 – 8 °C | ∞ | 1x |
**The annealing temperature depends on the melting temperature of the primers used
***The elongation time depends on the length of the fragments to be amplified. A time of 1 min/kb is recommended.
For optimal specificity and amplification an individual optimization of the recommended parameters may be necessary for each new template DNA and/or primer pair.
Produtos relacionados
Informações Detalhadas
Nosso tempo de faturamento é de até um dia útil durante nosso horário de funcionamento (Seg-Qui: 08:00 às 17:30 | Sex: 08:00 às 16:00). Caso a compra seja feita na sexta-feira, o faturamento só ocorrerá segunda-feira.
Produtos com disponibilidade IMEDIATA estão em nosso estoque próprio: Favor considerar 01 dia útil p/ faturamento + Tempo de transporte (a ser visualizado na página do produto)
Produtos que NÃO tenham disponibilidade IMEDIATA: Favor considerar Prazo em dias úteis informado em destaque + 01 dia útil p/ faturamento + Tempo de transporte (a ser visualizado na página do produto).
