Master mix Taq Pol (2X) Green | Master Mixes Cellco

Master mix Taq Pol (2X) Green

Ficha técnica

Código	Master mix Taq Pol (2X) Green
Categoria	PCR Convencional
Marca	Cellco

Descrição Geral

Master mix Taq Pol (2X) Green | Master Mixes - Cellco
Master mix para carregamento direto em géis de eletroforese
Pronto para uso, verde

For in vitro use only!

Envio: Shipped on blue ice

Condições de armazenamento: Store at -20 °C

Avoid freeze/thaw cycles. Taq Pol – Master mix (2X) is also stable for three months at 4°C, so for frequent use, an aliquot may be kept at 4°C.

Validade: 12 months

Kit contents:

2x Taq Pol Master Mix (purple cap)
Composition: 0.05 U/ µL Taq DNA polymerase, reaction buffer, 3 mM MgCl₂, 0.4 mM of each dNTP (dATP, dCTP, dGTP, dTTP), dye, 2 gel loading buffer and stabilizers.

Descrição:

Taq Master Mix contains Taq Polymerase in an optimized PCR buffer with Mg²⁺ and dNTPs. The master mix is supplemented with tracking dyes for direct loading of PCR products on gels. It contains all reagents required for PCR (except template and primer) in a premixed 2x concentrated ready-to-use solution. The Master Mix is recommended for use in routine PCR reactions. It is optimized for high specificity and guarantees minimal by-product formation. The tracking dyes in the master mix do not interfere with PCR performance and are compatible with downstream applications such as fluorescent automatic DNA sequencing, ligation, and restriction digestion. The blue dye migrates with 3-5 kb fragments and the yellow dye migrates faster than 20 bp (1% agarose gel).

PCR Reaction Setup:

Use the quantities below to prepare a single 50 µl PCR reaction. Thaw, mix, and briefly centrifuge each component before use. Add the following components to a microcentrifuge tube:

1. Prepare PCR master mix

Note: Consider the volumes for all components listed next steps to determine the correct amount of water required to reach your final reaction volume.

Component	50 μL rxn	Final conc.
Water, grade PCR	to 50 μl	-
2 X Taq Pol Master Mix	25 μl	1x

Mix and briefly centrifuge the components.

2. Add template DNA and primers

Component	50 μl rxn	final conc.
Forward primer (10 μM)	0,5 – 2,5 μl	0,1 – 0,5 μM
Reverse primer (10 μM)	0,5 – 2,5 μl	0,1 – 0,5 μM
Template DNA	x μl	10 pg – 1 μg*

*genomic DNA: 1 ng – 1 μg; plasmidial DNA: 1 pg – 1 ng

Cap each tube, mix, and briefly centrifuge the content.

3. Incubate reactions in a thermal cycler

Recommended cycling conditions:

Initial denaturation	95 °C	1 min	1x
Denaturation	95 °C	15 - 30 sec	30 cycles
Annealing**	45 - 68 °C	15 - 30 sec	30 cycles
Elongation***	72 °C	30 sec - 4 min	30 cycles
Final extension (optional)	72 °C	2 min	1x
Hold	4 – 8 °C	∞	1x

**The annealing temperature depends on the melting temperature of the primers used

***The elongation time depends on the length of the fragments to be amplified. A time of 1 min/kb is recommended.

For optimal specificity and amplification an individual optimization of the recommended parameters may be necessary for each new template DNA and/or primer pair.

Produtos visitados

Nenhum produto visitado

Informações Detalhadas

Nosso tempo de faturamento é de até um dia útil durante nosso horário de funcionamento (Seg-Qui: 08:00 às 17:30 | Sex: 08:00 às 16:00). Caso a compra seja feita na sexta-feira, o faturamento só ocorrerá segunda-feira.

Produtos com disponibilidade IMEDIATA estão em nosso estoque próprio: Favor considerar 01 dia útil p/ faturamento + Tempo de transporte (a ser visualizado na página do produto)

Produtos que NÃO tenham disponibilidade IMEDIATA: Favor considerar Prazo em dias úteis informado em destaque + 01 dia útil p/ faturamento + Tempo de transporte (a ser visualizado na página do produto).