Phi29 DNA Polymerase | Amplificação Genoma Completo (WGA) - Cellco
Bacillus subtilis phage phi29, recombinant, E. coli

For general laboratory use.

Definição de unidade: 
One unit is defined as the amount of the enzyme required to catalyze the incorporation of 10 nmol of dNTP into an acid-insoluble form in 30 minutes at 74 °C.

Envio: 
shipped on blue ice

Condições de armazenamento: 
store at -20 °C
avoid freeze/thaw cycles

Validade: 
6 months

Forma: 
liquid

Concentração: 
10 units/μl

Formulários:
Rolling-circle amplification (RCA)
Multiple displacement amplification (MDA)
Whole genome amplification (WGA)
Preparation of DNA template for sequencing

Descrição:
Phi29 DNA polymerase is a recombinant protein purified from E. coli cloned the gene encoding the DNA polymerase from Phi29 phage. Phi29 DNA polymerase is the replicative polymerase from the Bacillus subtilis phage Phi29 and possesses the highest processivity and strand-displacement activity among the known DNA polymerase.
Phi29 DNA polymerase contains a 3'→5' exonuclease activity that enables proofreading capability