Bst Polymerase 2.0 | Bst Polymerase - Cellco
Bst polimerase para amplificação isotérmica LAMP

For in vitro use only!

Shipping:
Shipped on blue ice Storage

Conditions:
Store at -20 °C
Avoid freeze/thaw cycles.

Shelf Life:
12 months

Concentration:
8 units/µL

Kit contents:
Bst 2.0 Polymerase (blue cap)
8 units/µl Bst DNA Polymerase in 10 mM Tris-HCl, 50 mM KCl, 50 % (v/v) Glycerol, pH 7.5 (25 °C) and stabilizers.

Isothermal Buffer (red cap) - 10x conc.
2200 mM Tris-HCl pH 8.8, KCl, NH₄)²SO₄, 20 mM MgSO4₄ and detergents.

MgSO₄ Stock Solution (yellow cap)
25 mM MgSO₄.

Description:
Bst 2.0 Polymerase is a genetically enhanced Bst polymerase of the next generation. The polymerase is the ideal choice for robust amplification of DNA at constant temperature (60 to 65 °C). The enzyme shows high strand displacement activity and generates an amplification factor of up to 10⁹ which is comparable to approx. 30 cycles in a PCR assay. The polymerase  allows detection of a target gene within 30-60 minutes.